

| Catalog No. | JN038018 |
|---|---|
| Description |
Treponema pallidum TPP47 ELISA Kit (JN038018) — Quantitative sandwich ELISA for Treponema pallidum TPP47. Sample: Plasma, Serum. Range: 1.56 - 100 ng/mL. Sensitivity: 0.65 ng/mL.
Highlights
|
| Sample type | Serum, plasma, and other biological fluid samples |
| Sensitivity | 0.71 ng/mL |
| Range | 1.56–100 ng/mL |
| Species reactivity | Treponema pallidum |
| Target | Treponema pallidum TPP47 |
| Applications | ELISA |
| Detection method | Colorimetric |
| Assay type | Sandwich |
| Recovery | 80-120% |
| Shipping | 2-8 ℃ |
| Stability and Storage | The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 10% prior to the expiration date under appropriate storage condition. |
| Specifications | TPP47 |
| Background | Putative DD-carboxypeptidase TP_0574 (TPP47) is a ~47 kDa protein. A possible D,D-carboxypeptidase, that releases amino acids sequentially from a proteins C-terminus. Has zinc-dependent carboxypeptidase activity on synthetic depsipeptide substrates. May serve to decrease cross-linking of peptidoglycan, promoting the highly sinusous motility of this spirochaete (Probable). Overexpression of the whole protein in E.coli leads to aberrant cell morphology and extrusion of the cytoplasm, while overexpression of a construct with the first 62 resides of the protein fused to PhoA does have this effect, suggesting the whole protein, not the lipoprotein moiety, is toxic. Binds penicillin. 1. Deka, RK. et al. (2002) The Journal of biological chemistry 277, 41857-64. PMID: 12196546 2. Weigel, LM. et al. (1994) Proceedings of the National Academy of Sciences of the United States of America 91, 11611-5. PMID: 7972112 3. Radolf, JD. et al. (1989) Infection and immunity 57, 1248-54. PMID: 2647634 |
| Note | For research use only. |
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