Tuberculosis Research Reagents — Mycobacterium tuberculosis Antibodies, Proteins & ELISA Kits

Tuberculosis (TB) remains one of the leading infectious disease killers globally, caused by Mycobacterium tuberculosis (Mtb). Accurate diagnosis depends on detecting secreted and surface antigens including ESAT-6, CFP-10, Ag85 complex, and MPT64 — the key immunodominant targets used in interferon-gamma release assays (IGRAs), rapid diagnostic tests, and vaccine research. The rising burden of drug-resistant TB (MDR-TB, XDR-TB) and TB-HIV coinfection has intensified demand for high-quality research reagents supporting diagnostic assay development, host-pathogen interaction studies, and next-generation vaccine candidates.

Research Use Only (RUO)Not intended for diagnostic or therapeutic procedures.

Tuberculosis research targets — ESAT-6, CFP-10, Ag85 complex, MPT64, host immune response to Mycobacterium tuberculosis

Fig. 1 Key immunological targets and diagnostic antigens in tuberculosis research. ESAT-6/CFP-10 secretion system, Ag85 complex, MPT64, and host T cell immune response. Key research targets highlighted in orange.

abinScience provides validated recombinant antibodies, polyclonal antibodies, and recombinant proteins for key M. tuberculosis research targets — including ESAT-6, CFP-10, Ag85, and MPT64. Products are validated for ELISA, WB, IHC, FCM, IF, and SPR. All manufactured by our parent company AtaGenix Laboratories under ISO 9001 & ISO 13485 quality systems. Contact us for custom antibody development.

Key Research Targets

ESAT-6 (EsxA) — Early Secretory Antigenic Target-6 is a 6 kDa protein secreted via the ESX-1 (Type VII) secretion system and is a primary virulence factor of M. tuberculosis. ESAT-6 forms a heterodimer with CFP-10 and is a key antigen in interferon-gamma release assays (IGRAs) such as QuantiFERON-TB Gold. Anti-ESAT-6 antibodies and recombinant proteins support IGRA development, T cell stimulation assays, and vaccine candidate evaluation.
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CFP-10 (EsxB) — Culture Filtrate Protein-10 is the binding partner of ESAT-6 in the ESX-1 secretion complex. The ESAT-6/CFP-10 heterodimer is central to Mtb pathogenesis and serves as a differential diagnostic antigen (absent in BCG vaccine strains and most non-tuberculous mycobacteria). Anti-CFP-10 antibodies support diagnostic assay development and host-pathogen interaction studies.
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Ag85 Complex (Ag85A/B/C) — The Antigen 85 complex comprises three mycolyl transferases (Ag85A, Ag85B, Ag85C) essential for mycobacterial cell wall biosynthesis. Ag85B is among the most abundant secreted proteins of Mtb and a leading tuberculosis vaccine candidate antigen (e.g., H4:IC31, M72/AS01E). Anti-Ag85 antibodies and recombinant proteins support vaccine immunogenicity studies, ELISA-based detection, and drug target research.
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MPT64 — MPT64 is an immunodominant secreted protein specific to the M. tuberculosis complex, widely used in rapid immunochromatographic tests (ICTs) for culture confirmation of Mtb. Anti-MPT64 antibodies enable point-of-care diagnostic assay development, IHC-based detection in tissue biopsies, and serodiagnostic research.
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References

1. World Health Organization. Global tuberculosis report 2024. Geneva: WHO; 2024. Link

2. Pai M, Denkinger CM, Kik SV, et al. Gamma interferon release assays for detection of Mycobacterium tuberculosis infection. Clin Microbiol Rev. 2014;27(1):3-20. DOI

3. Tameris MD, Hatherill M, Landry BS, et al. Safety and efficacy of MVA85A, a new tuberculosis vaccine, in infants previously vaccinated with BCG. Lancet. 2013;381(9871):1021-1028. DOI

4. Brodin P, Rosenkrands I, Andersen P, Cole ST, Brosch R. ESAT-6 proteins: protective antigens and virulence factors? Trends Microbiol. 2004;12(11):500-508. DOI

5. Tiwari D, et al. Key residues of the mycobacterial Ag85B antigen contribute to antimycobacterial activity. J Biol Chem. 2009;284(5):3069-3075. DOI