Anti-BCL2 Polyclonal Antibody (HY131014)

Datasheet Share product Copy info

Price(USD): $

Spec:

20ug
50ug
100ug

Number:

Overview
Images
References
Datasheet

Overview

Catalog No.

HY131014

Description

Anti-BCL2 Polyclonal Antibody (HY131014) is a rabbit polyclonal antibody detecting BCL2 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Dog.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat, Bos taurus, Dog, Cricetulus griseus

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human BCL2 (Met1-Asp211).

Target

Apoptosis regulator Bcl-2, BCL2

Purification

Purified by antigen affinity column.

Accession

P10415

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Apoptosis regulator Bcl-2 (BCL2) is a ~26 kDa protein. Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). Also acts as an inhibitor of autophagy: interacts with BECN1 and AMBRA1 during non-starvation conditions and inhibits their autophagy function.

1. Eguchi, Y. et al. (1992) Nucleic acids research 20, 4187-92. PMID: 1508712

2. Yin, XM. et al. (1994) Nature 369, 321-3. PMID: 8183370

3. Ruvolo, PP. et al. (2001) Leukemia 15, 515-22. PMID: 11368354

4. Wei, Y. et al. (2008) Molecular cell 30, 678-88. PMID: 18570871

5. Chen, D. et al. (2010) The Journal of biological chemistry 285, 38214-23. PMID: 20889974

6. Strappazzon, F. et al. (2011) The EMBO journal 30, 1195-208. PMID: 21358617

7. Bruey, JM. et al. (2007) Cell 129, 45-56. PMID: 17418785

References

1. Gracillin Protects Liver Ischemia-Reperfusion Injury from Oxidative Stress-Induced Apoptosis. [HY131014]

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-BCL2 polyclonal antibody at 1ug/mL on recombinant BCL2 (Catalog No. HY131012).
Western blot

Gracillin pretreatment significantly restored Bcl-2 expression suppressed by liver ischemia-reperfusion injury.