Mpox / Monkeypox Virus (MPXV) Research Reagents — Antibodies, Recombinant Proteins & ELISA Kits

Monkeypox virus (MPXV, recently renamed mpox virus) is a large, enveloped, double-stranded DNA orthopoxvirus (~197 kb genome) that causes mpox, a zoonotic disease endemic to Central and West Africa that became a global public health emergency in 2022 (WHO PHEIC, Clade IIb). MPXV exists in two clades — Clade I (Central African, higher virulence) and Clade II (West African, lower virulence) — with a 2023–2024 Clade Ib outbreak in the Democratic Republic of Congo raising additional pandemic concern. Like all orthopoxviruses, MPXV produces two infectious forms: intracellular mature virus (MV) and extracellular enveloped virus (EV). Key MV surface antigens include A29L (GAG-binding, homolog of VACV A27L), E8L, H3L, and L1R; EV surface antigens include A35R (homolog of VACV A33R), M1R, and B6R (complement control). Both MV and EV targets must be covered for complete neutralization.

Research Use OnlyNot for diagnostic or therapeutic use.

abinScience offers one of the most comprehensive MPXV/mpox reagent portfolios available — covering all major MV and EV surface antigens plus diagnostic and immune evasion targets — including recombinant antibodies, monoclonal antibodies, recombinant proteins, polyclonal detection antibodies, and ELISA kits for A29L, A35R, H3L, M1R, E8L, and B6R. Manufactured by AtaGenix Laboratories (ISO 9001 & ISO 13485). For VACV/cowpox cross-reactive reagents, see our Vaccinia virus page. Contact us for Clade I vs. Clade II-specific reagents and bulk orders.

MV Surface Antigens (Intracellular Mature Virus)

A29L — Primary MV Attachment Protein
A29L (homolog of VACV A27L) is a homotrimeric MV surface protein that mediates GAG-dependent cell attachment and is a major target for MV-neutralizing antibodies. A29L is the most immunodominant MPXV antigen and a key component of next-generation mpox vaccines. abinScience provides the deepest A29L antibody panel available: monoclonal antibodies 1A003 (VK415015), 1A004 (VK415025), 1A005 (VK415035); recombinant antibodies including SAA0289 (VK415013) and multiple additional clones; recombinant A29L proteins (C-His VK415011, N-His VK415012, N-GST VK415032); and anti-A29L pAb (VK415014).

H3L — MV Surface Glycoprotein
H3L (Ag35) binds heparan sulfate and is an abundant MV surface protein. Available: monoclonal antibodies 1A434 (VK707015) and 1A435 (VK707025), recombinant antibodies, recombinant H3L proteins, anti-H3L pAb (VK707014), and H3L ELISA Kit (VK707018).

E8L — MV Entry Protein
E8L is an MPXV-specific MV surface protein involved in viral entry. Available: recombinant antibody SAA0285 (VK680013), recombinant E8L proteins, anti-E8L pAb (VK680014), and E8L ELISA Kit (VK680018).

EV Surface Antigens (Extracellular Enveloped Virus)

A35R — EV Glycoprotein
A35R (homolog of VACV A33R) is an EV-specific surface glycoprotein required for efficient virus release and long-range dissemination. Anti-A35R antibodies limit EV-mediated spread and reduce disease severity in animal models. Available: recombinant antibody SAA0287 (VK601013) and additional clones; recombinant A35R proteins in multiple formats; anti-A35R pAb (VK601014); and A35R ELISA Kit (VK601018).

M1R — EV Surface Protein
M1R is an EV-specific surface protein unique to MPXV (no direct VACV homolog), making it a potential target for MPXV-specific diagnostics. Available: recombinant antibody SAA0283 (VK785013) and additional clones; recombinant M1R proteins; anti-M1R pAb (VK785014); and M1R ELISA Kit (VK785018).

B6R — Complement Control Protein
B6R (homolog of VACV B5R) is an EV surface complement regulatory protein that protects virions from complement-mediated neutralization. Available: recombinant antibody SAA2203 (VK526013) and additional clones; recombinant B6R proteins; anti-B6R pAb (VK526014); and B6R ELISA Kit (VK526018).

Additional MPXV Targets & Diagnostic Antigens

abinScience also provides reagents for MPXV immune evasion and replication proteins:
• A27L (GAG binding): protein N-His (VK708012) and pAb (VK708014)
• A33R (EV surface): protein N-His (VK469012) and pAb (VK469014)
• B5R (complement control): protein N-His (VK784012) and pAb (VK784014)
• B21R (secreted glycoprotein): protein and pAb
• A36R (actin tail formation): pAb (VK559014)
• D13L (rifampicin target, crescent membrane scaffold): pAb (VK741014)
• J2R (thymidine kinase, TPOXX/tecovirimat pathway): protein and pAb
• F13L (palmitoylated EV protein, tecovirimat target): protein and pAb

ELISA Kit Panel
Ready-to-use antigen-detection ELISA kits available for six key MPXV proteins: M1R, H3L, E8L, A35R, B6R, and additional targets. Each kit includes pre-optimized antibody pairs, calibrators, and substrates.

Frequently Asked Questions

Which targets should I prioritize for MPXV neutralization studies?
Like all orthopoxviruses, complete MPXV neutralization requires targeting both MV and EV forms. For MV: combine anti-A29L + anti-H3L or anti-E8L. For EV: add anti-A35R + anti-B6R. A four-antibody cocktail covering MV + EV achieves the broadest neutralization. Our A29L antibody panel alone offers the most extensive MV-neutralizing clone set.

How do your MPXV reagents relate to VACV reagents?
MPXV and VACV are closely related orthopoxviruses with homologous surface antigens: MPXV A29L ≈ VACV A27L, MPXV A35R ≈ VACV A33R, MPXV B6R ≈ VACV B5R. Cross-reactivity is expected for some antibodies targeting conserved epitopes. Our Vaccinia virus page has VACV-specific reagents and additional orthopoxvirus tools. Contact us for cross-reactivity testing data.

Do you have Clade I vs. Clade II-specific reagents?
Most of our recombinant proteins and antibodies are derived from Clade II (West African) sequences, consistent with the 2022 global outbreak strain. For Clade I-specific reagents, including Clade Ib (2023–2024 DRC outbreak), contact us — AtaGenix can produce custom Clade I-specific proteins from published sequences within 4–6 weeks.

Which antigen is best for MPXV-specific diagnostics (vs. other orthopoxviruses)?
M1R has no direct VACV homolog and shows the highest MPXV-specificity among our panel. The M1R ELISA Kit is recommended for MPXV-specific antigen detection when cross-reactivity with vaccinia-vaccinated sera must be minimized.

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