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Anti-Human Semaphorin 4D/CD100 Polyclonal Antibody (HV563014)

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Overview
Catalog No.HV563014
Description
Anti-CD100/SEMA4D Polyclonal Antibody (HV563014) is a rabbit polyclonal antibody detecting SEMA4D in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human CD100/SEMA4D (Phe502-Phe636).
Target A8, Semaphorin-4D, GR3, BB18, SEMAJ, C9orf164, CD100, SEMA4D
Purification Purified by antigen affinity column.
Accession Q92854
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Semaphorin-4D (CD100/SEMA4D) is a ~96 kDa protein. Cell surface receptor for PLXNB1 and PLXNB2 that plays an important role in cell-cell signaling. Regulates GABAergic synapse development. Promotes the development of inhibitory synapses in a PLXNB1-dependent manner. Modulates the complexity and arborization of developing neurites in hippocampal neurons by activating PLXNB1 and interaction with PLXNB1 mediates activation of RHOA. Promotes the migration of cerebellar granule cells.

1. Janssen, BJ. et al. (2010) Nature 467, 1118-22. PMID: 20877282
2. Vodrazka, P. et al. (2009) The European journal of neuroscience 30, 1193-208. PMID: 19788569
3. Basile, JR. et al. (2005) Molecular and cellular biology 25, 6889-98. PMID: 16055703
4. Hall, KT. et al. (1996) Proceedings of the National Academy of Sciences of the United States of America 93, 11780-5. PMID: 8876214
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human Semaphorin 4D/CD100 Polyclonal Antibody

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