Anti-RTCB Polyclonal Antibody (HV728014)

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Spec:

50ug
100ug

Number:

Overview
Images
References
Datasheet

Overview

Catalog No.

HV728014

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human RTCB (Met1-Gly505).

Target

C22orf28, RNA-splicing ligase RtcB homolog, RTCB, 3'-phosphate/5'-hydroxy nucleic acid ligase

Purification

Purified by antigen affinity column.

Accession

Q9Y3I0

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

For research use only.

Images

Immunofluorescence

A549 cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting RTCB (HV728014) at a concentration of 31.5 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of RTCB was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).
Western blot

Western blot analysis was performed using anti-RTCB polyclonal antibody at 1μg/ml on various samples.
Lane 1: 293T cell lysate
Lane 2: Jurkat cell lysate
Lane 3: Mouse liver lysate

References

Datasheet

Anti-RTCB Polyclonal Antibody.pdf

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Anti-RTCB Polyclonal Antibody (HV728014)

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