Anti-Mouse IL-2 Recombinant Antibody (S4B6) (MX011023)

Name: Anti-Mouse IL-2 Recombinant Antibody (S4B6)
Brand: abinScience
SKU: MX011023
Price: 110 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

MX011023

Species reactivity

Mouse

Applications

ELISA, FCM

Host species

Rat

Isotype

IgG2a, kappa

Clone ID

S4B6

Clonality

Monoclonal

Tested applications

Target

IL2, IL-2, Aldesleukin, Interleukin-2, TCGF, T-cell growth factor

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P04351

RRID

Anti-Mouse IL2 Antibody (S4B6) (abinScience Cat# MX011023, RRID:AB_3725657)

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Interleukin-2 (IL2) is a ~19 kDa protein. Cytokine produced by activated CD4-positive helper T-cells and to a lesser extend activated CD8-positive T-cells and natural killer (NK) cells that plays pivotal roles in the immune response and tolerance. Binds to a receptor complex composed of either the high-affinity trimeric IL-2R (IL2RA/CD25, IL2RB/CD122 and IL2RG/CD132) or the low-affinity dimeric IL-2R (IL2RB and IL2RG). Interaction with the receptor leads to oligomerization and conformation changes in the IL-2R subunits resulting in downstream signaling starting with phosphorylation of JAK1 and JAK3. In turn, JAK1 and JAK3 phosphorylate the receptor to form a docking site leading to the phosphorylation of several substrates including STAT5. This process leads to activation of several pathways including STAT, phosphoinositide-3-kinase/PI3K and mitogen-activated protein kinase/MAPK pathways.

1. Zhu, J. et al. (2003) Immunity 19, 739-48. PMID: 14614860

2. Reya, T. et al. (1998) Developmental immunology 5, 287-302. PMID: 9814585

3. Smith, GA. et al. (2016) Nature chemical biology 12, 373-9. PMID: 27018889

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Mouse IL2 Antibody (S4B6).
Western blot

Western blot analysis was performed using anti-IL2 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant mouse IL2 (Catalog No: MX011012)
Lane 2: negative control