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Anti-Influenza A virus Nucleoprotein Recombinant Antibody (SAA0410) (VK528013)

Anti-Influenza A virus Nucleoprotein Recombinant Antibody (SAA0410)
Anti-Influenza A virus Nucleoprotein Recombinant Antibody (SAA0410)
Anti-Influenza A virus Nucleoprotein Recombinant Antibody (SAA0410)
Anti-Influenza A virus Nucleoprotein Recombinant Antibody (SAA0410)
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Overview
Catalog No.VK528013
Description
Anti-Influenza A virus Nucleoprotein Recombinant Antibody (SAA0410) (VK528013) is a mouse monoclonal antibody detecting Nucleoprotein in ELISA, FCM, IF. Suitable for Influenza A virus (H1N1).
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityInfluenza A virus (H1N1)
ApplicationsELISA, FCM, IF
Host speciesMouse
ClonalityMonoclonal
IsotypeVHH-mFc
Clone IDSAA0410
Target Nucleoprotein, Nucleocapsid protein, Protein N, NP
Endotoxin level < 10 EU/mg
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession P15682
RRID Anti-Influenza A virus NP/Nucleoprotein Antibody (SAA0410) (abinScience Cat# VK528013, RRID:AB_3725949)
Form Liquid
Storage buffer 0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:2000-1:20000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background

Nucleoprotein (NP) is a ~56 kDa protein. Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the host nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals that are responsible for the active RNP import into the nucleus through cellular importin alpha/beta pathway. Later in the infection, nclear export of RNPs are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins.

Note For research use only.
Images
  • Anti-Influenza A virus Nucleoprotein Recombinant Antibody (SAA0410)

    SDS-PAGE

    SDS-PAGE for Anti-Influenza A virus NP/Nucleoprotein Antibody (SAA0410).

  • Anti-Influenza A virus Nucleoprotein Recombinant Antibody (SAA0410)

    Bioactivity

    Detects recombinant H1N1 nucleoprotein (Catalog No: VK528012) in indirect ELISA.

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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