Anti-Rat VEGFA Antibody (B20-4) (RB941013)

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Overview

Catalog No.	RB941013
Species reactivity	Human, Mouse, Rat, Rabbit
Applications	Blocking, ELISA
Host species	Mouse
Isotype	IgG2a, kappa
Clone ID	B20-4
Clonality	Monoclonal
Target	Vascular endothelial growth factor A, VPF, VEGFA, VEGF, Vascular permeability factor, VEGF-A
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P16612
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Note	For research use only.

Images

Bioactivity

Detects Rat VEGFA in indirect ELISAs.
SDS-PAGE

SDS-PAGE for Anti-Rat VEGFA Antibody (B20-4).
Flow CytoMetry

Flow-cytometry using FITC anti-human VEGF antibody. U87 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human VEGF monoclonal antibody (Catalog: RB941013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using PE anti-human VEGF antibody. U87 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human VEGF monoclonal antibody (Catalog: RB941013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet