Anti-Human PRMT3 IP Antibody (SAA0218) (HT002013)

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50ug
100ug
1mg

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Overview
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Datasheet

Overview

Catalog No.

HT002013

Description

Anti-Human PRMT3 IP Antibody (SAA0218) [SAA0218] (HT002013) is a mouse monoclonal antibody detecting PRMT3 in ELISA, IF, IP, WB. Suitable for Human.

Highlights

●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IF, IP, WB

Host species

Mouse

Isotype

IgG1

Clone ID

SAA0218

Clonality

Monoclonal

Target

PRMT3, HRMT1L3, Protein arginine N-methyltransferase 3, Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

O60678

RRID

Anti-Human PRMT3 IP Antibody (SAA0218) (abinScience Cat# HT002013, RRID:AB_3729752)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Protein arginine N-methyltransferase 3 (PRMT3) is a ~59 kDa protein. Protein-arginine N-methyltransferase that catalyzes both the monomethylation and asymmetric dimethylation of the guanidino nitrogens of arginine residues in target proteins, and therefore falls into the group of type I methyltransferases. Catalyzes the asymmetric arginine dimethylation at multiple sites in the Arg/Gly-rich region of small ribosomal subunit protein uS5/RPS2. Also appears to methylate other ribosomal proteins. May regulate retinoic acid synthesis and signaling by inhibiting ALDH1A1 retinal dehydrogenase activity. Contributes to methylation of histone H4 'Arg-3', a specific tag for epigenetic transcriptional activation.

1. Siarheyeva, A. et al. (2012) Structure (London, England : 1993) 20, 1425-35. PMID: 22795084

2. Liu, F. et al. (2013) Journal of medicinal chemistry 56, 2110-24. PMID: 23445220

3. Kaniskan, HÜ. et al. (2015) Angewandte Chemie (International ed. in English) 54, 5166-70. PMID: 25728001

4. Min, Z. et al. (2019) Cell death & disease 10, 581. PMID: 31378783

5. Verma, M. et al. (2021) Communications biology 4, 109. PMID: 33495566

6. Gupta, S. et al. (2024) The Biochemical journal 481, 1723-1740. PMID: 39513743

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Human PRMT3 IP Antibody (SAA0218)

References

Datasheet

Anti-Human PRMT3 IP Antibody (SAA0218).pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human PRMT3 IP Antibody (SAA0218) (HT002013)

Overview

Images

References

Datasheet

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