Anti-Human Integrin alpha 4/CD49d Recombinant Antibody (SRR0028) (HY596043)

Name: Anti-Human Integrin alpha 4/CD49d Recombinant Antibody (SRR0028)
Brand: abinScience
SKU: HY596043
Price: 191 USD
Availability: InStock

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Overview

Catalog No.	HY596043
Description	Anti-Human Integrin alpha 4/CD49d Recombinant Antibody (SRR0028) (HY596043) is a rabbit monoclonal antibody detecting Integrin alpha 4/CD49d in IF, FCM. Suitable for Human. Highlights ●Affinity Purified — Minimal background and high purity for reliable results. ●Multi-Application — Validated across multiple applications.
Species reactivity	Human
Applications	IF, FCM
Host species	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone ID	SRR0028
Tested applications	IF, FCM
Target	CD49 antigen-like family member D, CD49D, CD49d, ITGA4, Integrin alpha-4, Integrin alpha-IV, VLA-4 subunit alpha
Endotoxin level	< 10 EU/mg as determined by LAL method.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P13612
Form	Liquid
Storage buffer	0.01M PBS pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background	Integrin alpha-4 (CD49d/ITGA4) is a ~114 kDa protein. Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. 1. Fujita, M. et al. (2012) Journal of immunology (Baltimore, Md. : 1950) 189, 5809-19. PMID: 23125415 2. Saegusa, J. et al. (2008) The Journal of biological chemistry 283, 26107-15. PMID: 18635536 3. Fujita, M. et al. (2015) The Journal of biological chemistry 290, 259-71. PMID: 25398877 4. Morris, GE. et al. (2022) British journal of pharmacology 179, 4958-4973. PMID: 35802072
Note	For research use only.

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Immunofluorescence

Jurkat cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting Integrin alpha 4/CD49d (HY596043) at a concentration of 20 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) at a concentration of 6.5 ug/ml for 50 minutes at room temperature. The localization of Integrin alpha 4/CD49d was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).
Flow cytometry

Flow-cytometry using anti-Human CD49d antibody. Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an anti-Human CD49d monoclonal antibody (Catalog HY596043, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Rabbit IgG H&L Polyclonal Antibody, FITC (abinScience:TF690414) and cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet

Anti-Human Integrin alpha 4/CD49d Recombinant Antibody (SRR0028).pdf

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Recombinant Proteins

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Anti-Human Integrin alpha 4/CD49d Recombinant Antibody (SRR0028) (HY596043)

Overview

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References

Datasheet

SDS