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Anti-Human FOXP3 Monoclonal Antibody (1A209) (HV388025)

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Overview
Catalog No.HV388025
Species reactivityHuman
ApplicationsELISA, FCM, WB
Host speciesMouse
IsotypeIgG1
Clone ID1A209
Clonality Monoclonal
Immunogen Recombinant Human FOXP3 protein.
Target Forkhead box protein P3, Scurfin, Forkhead box protein P3, C-terminally processed, Forkhead box protein P3 41 kDa form, FOXP3, IPEX, JM2
Purification Protein A/G purified from cell culture supernatant.
Form Liquid
Storage buffer 0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Note For research use only.
Images
  • Flow Cytometry

    Flow-cytometry using anti-human FOXP3 antibody. Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human FOXP3 antibody monoclonal antibody (Catalog # HV388025, Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (MF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • Flow Cytometry

    Flow-cytometry using anti-human FOXP3 antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human FOXP3 antibody monoclonal antibody (Catalog # HV388025, Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (MF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • Flow CytoMetry

    Flow-cytometry using anti-human FOXP3 antibody. Jurkat cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an anti-human FOXP3 monoclonal antibody (Catalog: HV388025, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • Flow CytoMetry

    Flow-cytometry using anti-human FOXP3 antibody. Hela cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an anti-human FOXP3 monoclonal antibody (Catalog: HV388025, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.

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