3C Protease (VK794012)

Name: 3C Protease
Brand: abinScience
SKU: VK794012
Price: 298 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.	VK794012
Description	3C Protease (VK794012) expressed in E. coli. Highlights ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Protein length	3C Protease is cloned from Human rhinovirus, fused with GST tag and expressed in E.coli.
Applications	Remove fusion tags from proteins with the HRV 3C cleavage sequence(LEVLFQ↓GP).
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Form	Liquid
Storage buffer	50mM Tris-HCl, pH 8.0, 150mM NaCl, 10mM EDTA, 1mM DTT, 50% glycerol Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Note	For research use only.

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SDS-PAGE

SDS-PAGE for 3C Protease

Bioactivity

M: Marker
1: Control
2: Fusion protein digest with 3C protease

Case study		Formula
concentration	1 mg/ml	c mg/ml
volume	100 µl	V ml
substrate	100 µg	cV mg
10X Buffer	11 µl	V/9 ml
3C Protease	1 µl	cV/100 ml

Mix the components thoroughly and incubate the reaction at 4°C for 16 hours. After incubation, take 2–10 µg of the control and digested sample for analysis by SDS-PAGE.

References

Datasheet