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Anti-Human CD18/ITGB2 Antibody (SAA0815) (HY378107)

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Overview
Catalog No.HY378107
Species reactivityHuman
ApplicationsELISA, FCM, IHC, WB
Host speciesHuman
IsotypeIgG1
Clone IDSAA0815
Clonality Monoclonal
Target Complement receptor C3 subunit beta, Cell surface adhesion glycoproteins LFA-1/CR3/p150, 95 subunit beta, Integrin beta-2, MFI7, CD18, ITGB2, RHC13101
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession P05107
Form Liquid
Storage buffer 0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Note For research use only.
Images
  • Flow-cytometry

    Flow-cytometry using anti- human CD18 antibody.
    Human U-937cell line were stained with an irrelevant antibody (Gray Histogram) or an anti- human CD18 antibody monoclonal antibody (Catalog # HY378107 , Red Histogram) at a concentration of 20 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • Flow-cytometry

    Flow-cytometry using anti- human CD18 antibody.
    Human Jurkat cell line were stained with an irrelevant antibody (Gray Histogram) or an anti- human CD18 antibody monoclonal antibody (Catalog # HY378107 , Red Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • Bioactivity

    SEC-HPLC detection for Anti-Human CD18/ITGB2 Antibody (ABS0955).

  • Flow CytoMetry

    Flow-cytometry using anti-human CD18 antibody. U937 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD18 monoclonal antibody (Catalog: HY378107, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.

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