Anti-Human CD8a/Lyt2 Antibody (OKT8) (HF815207)

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Datasheet

Overview

Catalog No.	HF815207
Description	Anti-Human CD8a/Lyt2 Antibody (OKT8) [OKT8] (HF815207) is a mouse monoclonal antibody detecting CD8a in ELISA, FCM, IP. Suitable for Human. Highlights ●Multi-Application — Validated across multiple applications. ●Flow Cytometry — Validated for flow cytometry applications.
Species reactivity	Human
Applications	ELISA, FCM, IP
Host species	Mouse
Isotype	IgG2a kappa
Clone ID	OKT8
Clonality	Monoclonal
Target	T-cell surface glycoprotein CD8 alpha chain, T-cell surface glycoprotein Lyt-2, CD8a, Cd8a, Lyt-2, Lyt2
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P01732
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background	T-cell surface glycoprotein CD8 alpha chain (CD8a/Lyt2) is a ~25 kDa protein. Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex.
Note	For research use only.

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Flow-cytometry

Flow-cytometry using anti-human CD8a antibody.CD8a Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD8a antibody monoclonal antibody (Catalog # HF815207 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for Anti-Human CD8a/Lyt2 Antibody (OKT8)