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Research Grade Anti-Human CD50/ICAM3 (ICM3) (HW443013)

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Spec:
  • 100ug
  • 1mg
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  • References
  • Datasheet
Overview
Catalog No.HW443013
Species reactivityHuman
ApplicationsELISA, Bioactivity: FACS, Functional assay, Research in vivo
Host speciesHumanized
IsotypeIgG1
Clone IDICM3
Expression systemMammalian Cells
ClonalityMonoclonal
TargetCD50, CDw50, Intercellular adhesion molecule 3, ICAM-R, ICAM3, ICAM-3
Endotoxin levelPlease contact the lab for this information.
Purity>95% purity as determined by SDS-PAGE.
PurificationProtein A/G purified from cell culture supernatant.
AccessionP32942
FormLiquid
Storage buffer0.01M PBS, pH 7.4.
Stability and StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C for short-term storage (1-2 weeks). Store at -20°C for up to 12 months. For long-term storage, store at -80°C.
NoteFor research use only. Not suitable for clinical or therapeutic use.
Images
  • Bioactivity

    Detects Human CD50/ICAM3 in indirect ELISAs.

  • Bioactivity

    SEC-HPLC detection for Research Grade Anti-Human CD50/ICAM3 (ICM3).

  • SDS-PAGE

    SDS-PAGE for Research Grade Anti-Human CD50/ICAM3 (ICM3).

  • Flow CytoMetry

    Flow-cytometry using APC anti-human CD50 antibody. RPMI-8226 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD50 monoclonal antibody (Catalog: HW443013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

  • Flow CytoMetry

    Flow-cytometry using FITC anti-human CD50 antibody. RPMI-8226 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD50 monoclonal antibody (Catalog: HW443013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

  • Flow CytoMetry

    Flow-cytometry using PE anti-human CD50 antibody. RPMI-8226 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD50 monoclonal antibody (Catalog: HW443013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

  • Flow CytoMetry

    Flow-cytometry using anti-human CD50 antibody. U937 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD50 monoclonal antibody (Catalog: HW443013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.

References

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