Catalog No. | HF879016 |
---|---|
Species reactivity | Human |
Applications | ELISA, Bioactivity: FACS, Functional assay, Research in vivo |
Host species | Humanized |
Isotype | Fab'-IgG1-kappa |
Expression system | Mammalian Cells |
Clonality | Monoclonal |
Target | TNF, Tumor necrosis factor ligand superfamily member 2, N-terminal fragment, ICD2, NTF, TNF-a, TNF-alpha, Tumor necrosis factor, TNFSF2, TNFA, Cachectin, ICD1 |
Endotoxin level | Please contact the lab for this information. |
Purity | >95% purity as determined by SDS-PAGE. |
Purification | Protein L purified from cell culture supernatant. |
Accession | P01375 |
Form | Liquid |
Storage buffer | 0.01M PBS, pH 7.4. |
Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C for short-term storage (1-2 weeks). Store at -20°C for up to 12 months. For long-term storage, store at -80°C. |
Alternate Names | CDP870, PHA-738144, Certolizumabpegol, 428863-50-7 |
Background | Onercept is a recombinant soluble human tumor necrosis factor-alpha p55 receptor for use in Crohn's disease research. |
Note | For research use only. Not suitable for clinical or therapeutic use. |
Detects Human TNFa/TNF-alpha in indirect ELISAs.
SDS-PAGE for Research Grade Certolizumab.
Flow-cytometry using anti-human TNFa antibody. 293T transfected cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human TNFa monoclonal antibody (Catalog HF879016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
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