Research Grade Certolizumab (HF879016)

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100ug
1mg

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Overview
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Datasheet

Overview

Catalog No.	HF879016
Description	Certolizumab (HF879016) is a research-grade recombinant antibody targeting CD2. Produced in mammalian cells with native-like glycosylation. Highlights ●Research Grade — For PK/PD studies, assay development, and ADA research. ●Native Glycosylation — Mammalian expression ensures native-like patterns.
Species reactivity	Human
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Host species	Humanized
Isotype	Fab'-IgG1-kappa
Expression system	Mammalian Cells
Clonality	Monoclonal
Target	TNF, Tumor necrosis factor ligand superfamily member 2, N-terminal fragment, ICD2, NTF, TNF-a, TNF-alpha, Tumor necrosis factor, TNFSF2, TNFA, Cachectin, ICD1
Endotoxin level	Please contact the lab for this information.
Purity	>95% purity as determined by SDS-PAGE.
Purification	Protein L purified from cell culture supernatant.
Accession	P01375
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C for short-term storage (1-2 weeks). Store at -20°C for up to 12 months. For long-term storage, store at -80°C.
Alternate Names	CDP870, PHA-738144, Certolizumabpegol, 428863-50-7
Background	Tumor necrosis factor (TNFa/TNF-alpha) is a ~25 kDa protein. Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Up-regulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective. TNFa is the therapeutic target of adalimumab (Humira). 1. Nie, H. et al. (2013) Nature medicine 19, 322-8. PMID: 23396208 2. Friedmann, E. et al. (2006) Nature cell biology 8, 843-8. PMID: 16829952 3. Jinesh G, G. et al. (2012) Journal of leukocyte biology 92, 233-44. PMID: 22517918 4. Nakahara, H. et al. (2003) Arthritis and rheumatism 48, 1521-9. PMID: 12794819
Note	For research use only. Not suitable for clinical or therapeutic use.

Images

Bioactivity

Detects Human TNFa/TNF-alpha in indirect ELISAs.
SDS-PAGE

SDS-PAGE for Research Grade Certolizumab
Flow Cytometry

Flow-cytometry using anti-human TNFa antibody. 293T transfected cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human TNFa monoclonal antibody (Catalog HF879016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry

Flow-cytometry using APC anti-human TNFa antibody. 293T transfected cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human TNFa monoclonal antibody (Catalog HF879016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry

Flow-cytometry using PE anti-human TNFa antibody. 293T transfected cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human TNFa monoclonal antibody (Catalog HF879016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry

Flow-cytometry using FITC anti-human TNFa antibody. 293T transfected cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human TNFa monoclonal antibody (Catalog HF879016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.