





| Catalog No. | HF879016 | 
|---|---|
| Species reactivity | Human | 
| Applications | ELISA, Bioactivity: FACS, Functional assay, Research in vivo | 
| Host species | Humanized | 
| Isotype | Fab'-IgG1-kappa | 
| Expression system | Mammalian Cells | 
| Clonality | Monoclonal | 
| Target | TNF, Tumor necrosis factor ligand superfamily member 2, N-terminal fragment, ICD2, NTF, TNF-a, TNF-alpha, Tumor necrosis factor, TNFSF2, TNFA, Cachectin, ICD1 | 
| Endotoxin level | Please contact the lab for this information. | 
| Purity | >95% purity as determined by SDS-PAGE. | 
| Purification | Protein L purified from cell culture supernatant. | 
| Accession | P01375 | 
| Form | Liquid | 
| Storage buffer | 0.01M PBS, pH 7.4.                                                 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.  | 
                                        
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C for short-term storage (1-2 weeks). Store at -20°C for up to 12 months. For long-term storage, store at -80°C. | 
| Alternate Names | CDP870, PHA-738144, Certolizumabpegol, 428863-50-7 | 
| Background | Onercept is a recombinant soluble human tumor necrosis factor-alpha p55 receptor for use in Crohn's disease research. | 
| Note | For research use only. Not suitable for clinical or therapeutic use. | 

Detects Human TNFa/TNF-alpha in indirect ELISAs.

SDS-PAGE for Research Grade Certolizumab

Flow-cytometry using anti-human TNFa antibody. 293T transfected cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human TNFa monoclonal antibody (Catalog HF879016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
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