Anti-Human Coagulation factor III/Tissue factor Reference Antibody (Tisotumab, RUO) (HB058016)

Name: Anti-Human Coagulation factor III/Tissue factor Reference Antibody (Tisotumab, RUO)
Brand: abinScience
SKU: HB058016
Price: 176 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.	HB058016
Description	Tisotumab (HB058016) is a research-grade recombinant antibody targeting CD142. Produced in mammalian cells with native-like glycosylation. Highlights ●Research Grade — For PK/PD studies, assay development, and ADA research. ●Native Glycosylation — Mammalian expression ensures native-like patterns.
Species reactivity	Human
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Host species	Human
Isotype	IgG1-kappa
Expression system	Mammalian cells
Clonality	Monoclonal
Target	Tissue factor, CD142, F3, Thromboplastin, Coagulation factor III, TF
Endotoxin level	Please contact the lab for this information.
Purity	>95% purity as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P13726
Form	Liquid
Storage buffer	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	HuMax-TF-ADC, TF-011-MMAE, HuMax-TF, 1418628-81-5
Background	Tissue factor (CD142/F3) is a ~33 kDa protein. Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited proteolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade. CD142 is the therapeutic target of tisotumab vedotin (Tivdak).
Note	For research use only. Not suitable for clinical or therapeutic use.

Images

SEC-HPLC

SEC-HPLC detection for Research Grade Tisotumab.
Bioactivity

Detects CD142/F3/TF in indirect ELISAs.
SDS-PAGE

SDS-PAGE for Research Grade Tisotumab
Flow CytoMetry

Flow-cytometry using anti-human CD142 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD142 monoclonal antibody (Catalog: HB058016) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-CD142/F3/TF monoclonal antibody at 1μg/ml on various samples.
Lane 1: recombinant human CD142/F3/TF (Catalog No: HB058011), 50ng
Lane 2: recombinant human CD142/F3/TF (Catalog No: HB058011), 30ng
Lane 3: recombinant human CD142/F3/TF (Catalog No: HB058011), 20ng
Lane 4: recombinant human CD142/F3/TF (Catalog No: HB058011), 10ng
Flow cytometry

Flow-cytometry using APC anti-human CD142 antibody. A431 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD142 monoclonal antibody (Catalog HB058016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry

Flow-cytometry using PE anti-human CD142 antibody. A431 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD142 monoclonal antibody (Catalog HB058016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry

Flow-cytometry using FITC anti-human CD142 antibody. A431 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD142 monoclonal antibody (Catalog HB058016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.