| Catalog No. | VK794012 |
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| Description | 3C protease is a cysteine protease that recognizes the cleavage site of Leu-Glu-Val-Leu-Phe-Gln*Gly-Pro.It used to remove fusion tags from proteins with the HRV 3C cleavage sequence and is dual tagged for easy removal from the sample after cleavage.
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Activity definition: 1 unit (U) is defined as the amount of enzyme that cleaves at least 95% of the Cleavage Control Fusion Protein (100 μg in 1X HRV 3C Cleavage Buffer) in 16 hours at 4°C. |
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| Expression system | E. coli |
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| Protein length | 3C Protease is cloned from Human rhinovirus, fused with GST tag and expressed in E.coli. |
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| Applications | Remove fusion tags from proteins with the HRV 3C cleavage sequence(LEVLFQ↓GP). |
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| Species | HRV-14 |
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| Predicted molecular weight | 47.46 kDa |
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| Nature | Recombinant |
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| Endotoxin level | Please contact with the lab for this information. |
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| Purity | >95% as determined by SDS-PAGE. |
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| Form | Liquid |
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| Storage buffer | 50mM Tris-HCl, pH 8.0, 150mM NaCl, 10mM EDTA, 1mM DTT, 50% glycerol |
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| Shipping | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
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| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles.Store at 2 to 8 °C for one week .Store at -20 to -80 °C for twelve months from the date of receipt. |
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| Note | For research use only. |
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