Human ARG2 Recombinant Protein (N-His) (HX072012)

Name: Human ARG2 Recombinant Protein (N-His)
Brand: abinScience
SKU: HX072012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HX072012
Description	Recombinant Human ARG2 Protein, N-His (HX072012) expressed in E. coli, spanning Val23-Ile354. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P78540
Protein length	Val23-Ile354
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	38.38 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Arginase-2, mitochondrial, Type II arginase, Kidney-type arginase, Arginase II, Non-hepatic arginase, ARG2
Background	Arginase-2, mitochondrial (ARG2/Arginase II) is a ~38 kDa protein. May play a role in the regulation of extra-urea cycle arginine metabolism and also in down-regulation of nitric oxide synthesis. Extrahepatic arginase functions to regulate L-arginine bioavailability to nitric oxid synthase (NOS). Arginine metabolism is a critical regulator of innate and adaptive immune responses. Seems to be involved in negative regulation of the survival capacity of activated CD4(+) and CD8(+) T cells. May suppress inflammation-related signaling in asthmatic airway epithelium. 1. Geiger, R. et al. (2016) Cell 167, 829-842.e13. PMID: 27745970 2. Xu, W. et al. (2016) The Journal of clinical investigation 126, 2465-81. PMID: 27214549 3. McGovern, N. et al. (2017) Nature 546, 662-666. PMID: 28614294 4. Yepuri, G. et al. (2012) Aging cell 11, 1005-16. PMID: 22928666 5. Xiong, Y. et al. (2014) Autophagy 10, 2223-38. PMID: 25484082 6. Xiong, Y. et al. (2013) Journal of the American Heart Association 2, e000096. PMID: 23832324 7. Cama, E. et al. (2003) Biochemistry 42, 8445-51. PMID: 12859189
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human ARG2 Protein, N-His
Western blot

Detected by Anti-ARG2 monoclonal antibody (Catalog No: FP447016)

References

Datasheet

Human ARG2 Recombinant Protein (N-His).pdf

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Formula

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Human ARG2 Recombinant Protein (N-His) (HX072012)

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