Anti-Human ARG2/Arginase II Recombinant Antibody (C0020187) (HX072013)

Name: Anti-Human ARG2/Arginase II Recombinant Antibody (C0020187)
Brand: abinScience
SKU: HX072013
Price: 126 USD
Availability: InStock

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Overview

Catalog No.	HX072013
Description	Anti-Human ARG2/Arginase II Recombinant Antibody (C0020187) (HX072013) is a mouse monoclonal antibody detecting ARG2/Arginase II in BIF, IF. Suitable for Human. Highlights ●Affinity Purified — Minimal background and high purity for reliable results. ●Multi-Application — Validated across multiple applications.
Species reactivity	Human
Applications	BIF, IF
Host species	Mouse
Clonality	Monoclonal
Isotype	IgG2a, kappa
Clone ID	C0020187
Tested applications	WB, FCM
Target	Arginase-2, mitochondrial, Type II arginase, Kidney-type arginase, Arginase II, Non-hepatic arginase, ARG2
Endotoxin level	< 10 EU/mg as determined by LAL method.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P78540
RRID	Anti-Human ARG2/Arginase II Antibody (C0020187) (abinScience Cat# HX072013, RRID:AB_3725840)
Form	Liquid
Storage buffer	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background	Arginase-2, mitochondrial (ARG2/Arginase II) is a ~38 kDa protein. May play a role in the regulation of extra-urea cycle arginine metabolism and also in down-regulation of nitric oxide synthesis. Extrahepatic arginase functions to regulate L-arginine bioavailability to nitric oxid synthase (NOS). Arginine metabolism is a critical regulator of innate and adaptive immune responses. Seems to be involved in negative regulation of the survival capacity of activated CD4(+) and CD8(+) T cells. May suppress inflammation-related signaling in asthmatic airway epithelium. 1. Geiger, R. et al. (2016) Cell 167, 829-842.e13. PMID: 27745970 2. Xu, W. et al. (2016) The Journal of clinical investigation 126, 2465-81. PMID: 27214549 3. McGovern, N. et al. (2017) Nature 546, 662-666. PMID: 28614294 4. Yepuri, G. et al. (2012) Aging cell 11, 1005-16. PMID: 22928666 5. Xiong, Y. et al. (2014) Autophagy 10, 2223-38. PMID: 25484082 6. Xiong, Y. et al. (2013) Journal of the American Heart Association 2, e000096. PMID: 23832324 7. Cama, E. et al. (2003) Biochemistry 42, 8445-51. PMID: 12859189
Note	For research use only.
Isotype Control	Mouse IgG2a (Fc Silence) Recombinant Isotype Control (MOPC 173) (MF533023)

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Bioactivity

Detects Human ARG2/Arginase II in indirect ELISAs.
SDS-PAGE

SDS-PAGE for Anti-Human ARG2/Arginase II Antibody (C0020187).
Flow CytoMetry

Flow-cytometry using PE anti-human ARG2 antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human ARG2 monoclonal antibody (Catalog: HX072013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-ARG2 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human ARG2 (Catalog No: HX072012)
Lane 2: negative control

References

Datasheet

Anti-Human ARG2/Arginase II Recombinant Antibody (C0020187).pdf

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Anti-Human ARG2/Arginase II Recombinant Antibody (C0020187) (HX072013)

Overview

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References

Datasheet

SDS