Human RAD51AP1 Recombinant Protein (N-GST & C-His) (HP402022)

Name: Human RAD51AP1 Recombinant Protein (N-GST & C-His)
Brand: abinScience
SKU: HP402022
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HP402022
Description	Recombinant Human RAD51AP1 Protein, N-GST & C-His (HP402022) expressed in E. coli, spanning Arg70-Asn116. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q96B01
Protein length	Arg70-Asn116
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	33.29 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	HsRAD51AP1, PIR51, RAD51-associated protein 1, RAD51-interacting protein, RAD51AP1
Background	RAD51-associated protein 1 is a ~38 kDa protein. Structure-specific DNA-binding protein involved in DNA repair by promoting RAD51-mediated homologous recombination. Acts by stimulating D-Loop formation by RAD51: specifically enhances joint molecule formation through its structure-specific DNA interaction and its interaction with RAD51. Binds single-stranded DNA (ssDNA), double-stranded DNA (dsDNA) and secondary DNA structures, such as D-loop structures: has a strong preference for branched-DNA structures that are obligatory intermediates during joint molecule formation. Cooperates with WDR48/UAF1 to stimulate RAD51-mediated homologous recombination: both WDR48/UAF1 and RAD51AP1 have coordinated role in DNA-binding during homologous recombination and DNA repair. WDR48/UAF1 and RAD51AP1 also have a coordinated role in DNA-binding to promote USP1-mediated deubiquitination of FANCD2. 1. Modesti, M. et al. (2007) Molecular cell 28, 468-81. PMID: 17996710 2. Wiese, C. et al. (2007) Molecular cell 28, 482-90. PMID: 17996711 3. Dray, E. et al. (2010) Nature structural & molecular biology 17, 1255-9. PMID: 20871616 4. Parplys, AC. et al. (2014) DNA repair 24, 87-97. PMID: 25288561 5. Parplys, AC. et al. (2015) Nucleic acids research 43, 9817-34. PMID: 26323318 6. Dunlop, MH. et al. (2012) The Journal of biological chemistry 287, 12343-7. PMID: 22375013
Note	For research use only

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Datasheet

Human RAD51AP1 Recombinant Protein (N-GST & C-His).pdf

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Human RAD51AP1 Recombinant Protein (N-GST & C-His) (HP402022)

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