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GMP Grade Human Insulin (Tag Free) (HF990011)

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Overview
Catalog No.HF990011
Expression systemMammalian cells
AccessionP01308
Protein lengthαlpha chain:G-I-V-E-Q-C-C-T-S-I-C-S-L-Y-Q-L-E-N-Y-C-N beta chain:F-V-N-Q-H-L-C-G-S-H-L-V-E-A-L-Y-L-V-C-G-E-R-G-F-F-Y-T-P-K-T
ApplicationsELISA, Functional assay
SpeciesHomo sapiens (Human)
Biological activity Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED50 for this effect ≤60 ng/mL.
Nature Recombinant
Endotoxin level <0.01 EU/ug
Purity >95% as determined by SDS-PAGE.
Form Lyophilized
Reconstitution It is recommended to redissolve in sterile 0.01 M HCl at a concentration of not less than 1mg/mL.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesINS, Insulin, Insulin A chain, Insulin B chain
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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