InVivoMAb Anti-Mouse IFN-gamma Antibody (R4-6A2) (MF813020)

Name: InVivoMAb Anti-Mouse IFN-gamma Antibody (R4-6A2)
Brand: abinScience
SKU: MF813020
Price: 370 USD
Availability: InStock

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Notice · Catalog MF813020 has been discontinued.

Price(USD):

Spec:

100ug
1mg

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

MF813020

Description

InVivoMAb Anti-Mouse IFN-gamma Antibody (R4-6A2) (MF813020) is a rat monoclonal antibody detecting IFN-gamma in ELISA, FACS, Functional assay, Research in vivo. Suitable for Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Mouse

Applications

in vivo IFNγ neutralization, in vitro IFNγ neutralization

Host species

Rat

Clonality

Monoclonal

Isotype

IgG1, kappa

Clone ID

R4-6A2

Target

Interferon gamma, IFN-gamma, Immune interferon, IFNG

Endotoxin level

< 1 EU/mg as determined by LAL method.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P01580

Form

Liquid

Storage buffer

0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Interferon gamma (Interferon gamma,IFN-gamma,Immune interferon,IFNG) is a ~19 kDa protein. Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation. Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription. Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits.

1. El Bougrini, J. et al. (2006) Cellular and molecular biology (Noisy-le-Grand, France) 52, 9-15. PMID: 16914093

2. Hisamatsu, H. et al. (1996) The Journal of experimental medicine 183, 1807-16. PMID: 8666937

3. Greenlund, AC. et al. (1993) The Journal of biological chemistry 268, 18103-10. PMID: 8349687

4. Akiyama, K. et al. (1994) FEBS letters 343, 85-8. PMID: 8163024

5. Fabunmi, RP. et al. (2001) Journal of cell science 114, 29-36. PMID: 11112687

6. Lah, TT. et al. (1995) FEBS letters 363, 85-9. PMID: 7729559

Note

For research use only

Images

References

Datasheet