Anti-CCL11/Eotaxin Polyclonal Antibody (HW762014)

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50ug
100ug

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HW762014

Description

Anti-CCL11/Eotaxin Polyclonal Antibody (HW762014) is a rabbit polyclonal antibody detecting Eotaxin-1 in ELISA, IHC, WB. Suitable for Human and Macaca mulatta.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human, Macaca mulatta

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CCL11/Eotaxin (Gly24-Pro97).

Target

Small-inducible cytokine A11, SCYA11, Eotaxin, CCL11, Eosinophil chemotactic protein, C-C motif chemokine 11, Eotaxin1, Eotaxin-1

Purification

Purified by antigen affinity column.

Accession

P51671

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Eotaxin (CCL11) is a ~10 kDa protein. Chemokine that plays a central role in both allergic and non-allergic inflammatory reactions by inducing the migration of different leukocyte types including eosinophils, basophils, macrophages and dendritic cells. Exerts its effects primarily by binding to the CCR3 receptor. Induces also chemotaxis of endothelial cells and promotes angiogenesis by activating the PI3K/Akt pathway.

1. Garcia-Zepeda, EA. et al. (1996) Nature medicine 2, 449-56. PMID: 8597956

2. Wakabayashi, K. et al. (2021) Scientific reports 11, 7937. PMID: 33846499

3. Kitaura, M. et al. (1996) The Journal of biological chemistry 271, 7725-30. PMID: 8631813

4. Park, JY. et al. (2017) Journal of receptor and signal transduction research 37, 416-421. PMID: 28279120

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-CCL11/Eotaxin polyclonal antibody at 1ug/mL on recombinant CCL11/Eotaxin (Catalog No. HW762012).

References

Datasheet