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Anti-Nectin-2/CD112 Polyclonal Antibody (HV456014)

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Overview
Catalog No.HV456014
Description
Anti-CD112/Nectin-2/NECTIN2 Polyclonal Antibody (HV456014) is a rabbit polyclonal antibody detecting Nectin-2 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human CD112/Nectin-2/NECTIN2 (Gln32-Pro350).
Target Herpesvirus entry mediator B, Herpes virus entry mediator B, Nectin cell adhesion molecule 2, HVEB, PVRL2, Poliovirus receptor-related protein 2, CD112, PRR2, Nectin-2, HveB, NECTIN2
Purification Purified by antigen affinity column.
Accession Q92692
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Nectin-2 (CD112/PVRL2) is a ~57 kDa protein. Modulator of T-cell signaling. Can be either a costimulator of T-cell function, or a coinhibitor, depending on the receptor it binds to. Upon binding to CD226, stimulates T-cell proliferation and cytokine production, including that of IL2, IL5, IL10, IL13, and IFNG. Upon interaction with PVRIG, inhibits T-cell proliferation. These interactions are competitive.

1. Zhu, Y. et al. (2016) The Journal of experimental medicine 213, 167-76. PMID: 26755705
2. Warner, MS. et al. (1998) Virology 246, 179-89. PMID: 9657005
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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