Anti-CD133/PROM1 Polyclonal Antibody (HT495014)

Name: Anti-CD133/PROM1 Polyclonal Antibody
Brand: abinScience
SKU: HT495014
Price: 168 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HT495014

Description

Anti-CD133/PROM1 Polyclonal Antibody (HT495014) is a rabbit polyclonal antibody detecting PROM1 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CD133/PROM1 (Leu805-Ans865).

Target

PROML1, PROM1, Prominin-1, Antigen AC133, CD133, Prominin-like protein 1

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

O43490

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Prominin-1 (CD133/PROM1) is a ~97 kDa protein. May play a role in cell differentiation, proliferation and apoptosis. Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner.

1. Mak, AB. et al. (2014) Journal of molecular biology 426, 2175-82. PMID: 24556617

2. Takenobu, H. et al. (2011) Oncogene 30, 97-105. PMID: 20818439

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-CD133/PROM1 polyclonal antibody at 1ug/mL on recombinant CD133/PROM1 (Catalog No. HT495022).

References

Datasheet