Anti-Human CD133 Recombinant Antibody (SAA0050) (HT495107)

Name: Anti-Human CD133 Recombinant Antibody (SAA0050)
Brand: abinScience
SKU: HT495107
Price: 191 USD
Availability: InStock

Price(USD):

Spec:

Number:

Get quote

Overview

Catalog No.	HT495107
Species reactivity	Human
Applications	FCM
Host species	Human
Isotype	IgG1
Clone ID	SAA0050
Clonality	Monoclonal
Target	PROML1, PROM1, Prominin-1, Antigen AC133, CD133, Prominin-like protein 1
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	O43490
Form	Liquid
Storage buffer	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background	Prominin-1 (CD133/PROM1) is a ~97 kDa protein. May play a role in cell differentiation, proliferation and apoptosis. Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner. 1. Mak, AB. et al. (2014) Journal of molecular biology 426, 2175-82. PMID: 24556617 2. Takenobu, H. et al. (2011) Oncogene 30, 97-105. PMID: 20818439
Note	For research use only.

Images

Flow-cytometry

Flow-cytometry using anti- human CD133 antibody.
Human CACO-2 cell line were stained with an irrelevant antibody (Gray Histogram) or an anti- human CD133 antibody monoclonal antibody (Catalog # HT495107 , Red Histogram) at a concentration of 20 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414 ) and cells analysed on a NovoCyte Flow Cytometer.
Bioactivity

SEC-HPLC detection for Anti-Human CD133/PROM1 Antibody (ABS1140).