Anti-CD142/F3/TF Polyclonal Antibody (HB058014)

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50ug
100ug

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HB058014

Description

Anti-CD142/F3/TF Polyclonal Antibody (HB058014) is a rabbit polyclonal antibody detecting CD142 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CD142/F3/TF (Met1-Glu251).

Target

Tissue factor, CD142, F3, Thromboplastin, Coagulation factor III, TF

Purification

Purified by antigen affinity column.

Accession

P13726

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Tissue factor (CD142/F3) is a ~33 kDa protein. Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited proteolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade. CD142 is the therapeutic target of tisotumab vedotin (Tivdak).

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-CD142/F3/TF polyclonal antibody at 1ug/mL on recombinant CD142/F3/TF (Catalog No. HB058012).

References

Datasheet