Anti-CD178/FASLG/TNFSF6 Polyclonal Antibody (HW497014)

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50ug
100ug

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Overview
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Datasheet
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Overview

Catalog No.

HW497014

Description

Anti-CD178/FASLG/TNFSF6 Polyclonal Antibody (HW497014) is a rabbit polyclonal antibody detecting TNFSF6 in ELISA, IHC, WB. Suitable for Human, Cat, Cercocebus atys, Macaca fascicularis, and Macaca mulatta.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Cat, Cercocebus atys, Macaca fascicularis, Macaca mulatta, Macaca nemestrina, Pig

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CD178/FASLG/TNFSF6 (Gln103-Leu281).

Target

Receptor-binding FasL ectodomain, APL, APT1LG1, FasL ICD, Fas antigen ligand, CD178, sFasL, CD95L, APTL, TNFSF6, Apoptosis antigen ligand, SPA, FASLG, FasL, SPPL2A-processed FasL form, Tumor necrosis factor ligand superfamily member 6, Soluble Fas ligand, Fas ligand, CD95 ligand, CD95-L, FASL

Purification

Purified by antigen affinity column.

Accession

P48023

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Tumor necrosis factor ligand superfamily member 6 (CD178/FASLG) is a ~31 kDa protein. Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. Involved in cytotoxic T-cell-mediated apoptosis, natural killer cell-mediated apoptosis and in T-cell development. Initiates fratricidal/suicidal activation-induced cell death (AICD) in antigen-activated T-cells contributing to the termination of immune responses. TNFRSF6/FAS-mediated apoptosis also has a role in the induction of peripheral tolerance. Binds to TNFRSF6B/DcR3, a decoy receptor that blocks apoptosis.

1. Ruiz-García, R. et al. (2015) Pediatric research 78, 603-8. PMID: 26334989

2. Schneider, P. et al. (1997) The Journal of biological chemistry 272, 18827-33. PMID: 9228058

3. Alderson, MR. et al. (1995) The Journal of experimental medicine 181, 71-7. PMID: 7528780

4. Tanaka, M. et al. (1998) Nature medicine 4, 31-6. PMID: 9427603

5. Liu, W. et al. (2016) Structure (London, England : 1993) 24, 2016-2023. PMID: 27806260

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-CD178/FASLG/TNFSF6 polyclonal antibody at 1ug/mL on recombinant CD178/FASLG/TNFSF6 (Catalog No. HW497012).

References

Datasheet