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Anti-Human ASTL/Ovastacin Recombinant Antibody (SAA1599) (HP237013)

Anti-Human ASTL/Ovastacin Recombinant Antibody (SAA1599)
Anti-Human ASTL/Ovastacin Recombinant Antibody (SAA1599)
Anti-Human ASTL/Ovastacin Recombinant Antibody (SAA1599)
Anti-Human ASTL/Ovastacin Recombinant Antibody (SAA1599)
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Overview
Catalog No.HP237013
Species reactivityHuman
ApplicationsELISA
Host speciesMouse
IsotypeIgG2a, kappa
Clone IDSAA1599
Clonality Monoclonal
Target Oocyte astacin, Astacin-like metalloendopeptidase, ZP2-proteinase, ASTL, Ovastacin
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession Q6HA08
RRID Anti-Human ASTL/Ovastacin Antibody (SAA1599) (abinScience Cat# HP237013, RRID:AB_3727360)
Form Liquid
Storage buffer 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:2000-1:20000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background

Astacin-like metalloendopeptidase (ASTL/Ovastacin) is a ~45 kDa protein. Oocyte-specific oolemmal receptor involved in sperm and egg adhesion and fertilization. Plays a role in the polyspermy inhibition. Probably acts as a protease for the post-fertilization cleavage of ZP2. Cleaves the sperm-binding ZP2 at the surface of the zona pellucida after fertilization and cortical granule exocytosis, rendering the zona pellucida unable to support further sperm binding.

Note For research use only.
Images
  • Anti-Human ASTL/Ovastacin Recombinant Antibody (SAA1599)

    Bioactivity

    Detects Recombinant Human ASTL/Ovastacin Protein, C-His (Cat No.: HP237011) in indirect ELISA.

  • Anti-Human ASTL/Ovastacin Recombinant Antibody (SAA1599)

    SDS-PAGE

    SDS-PAGE for Anti-Human ASTL/Ovastacin Antibody (SAA1599).

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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