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Anti-Human ASTL/Ovastacin Monoclonal Antibody (1A069) (HP237025)

Anti-Human ASTL/Ovastacin Monoclonal Antibody (1A069)
Anti-Human ASTL/Ovastacin Monoclonal Antibody (1A069)
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Overview
Catalog No.HP237025
Description
Anti-Human ASTL/Ovastacin Monoclonal Antibody (1A069) [1A069] (HP237025) is a mouse monoclonal antibody detecting Ovastacin in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Recombinant — Superior batch-to-batch reproducibility with defined sequences.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesMouse
ClonalityMonoclonal
IsotypeIgG1
Clone ID1A069
Immunogen Mammalian cells - derived recombinant Human ASTL / Ovastacin (Met1-Asp431).
Target Oocyte astacin, Astacin-like metalloendopeptidase, ZP2-proteinase, ASTL, Ovastacin
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession Q6HA08
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Astacin-like metalloendopeptidase (ASTL/Ovastacin) is a ~45 kDa protein. Oocyte-specific oolemmal receptor involved in sperm and egg adhesion and fertilization. Plays a role in the polyspermy inhibition. Probably acts as a protease for the post-fertilization cleavage of ZP2. Cleaves the sperm-binding ZP2 at the surface of the zona pellucida after fertilization and cortical granule exocytosis, rendering the zona pellucida unable to support further sperm binding.

Note For research use only.
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  • Anti-Human ASTL/Ovastacin Monoclonal Antibody (1A069)

    SDS-PAGE

    SDS-PAGE for Anti-Human ASTL/Ovastacin Monoclonal Antibody (1A069)

Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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