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Anti-Human Tryptase alpha/beta 1 Recombinant Antibody (E104.v2) (HS931023)

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Overview
Catalog No.HS931023
Description
Anti-Human Tryptase alpha/beta 1 Recombinant Antibody (E104.v2) (HS931023) is a human monoclonal antibody detecting Tryptase alpha/beta 1 in ELISA. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
Species reactivityHuman
ApplicationsELISA
Host speciesHuman
ClonalityMonoclonal
IsotypeIgG1, kappa
Clone IDE104.v2
Target EC:3.4.21.59, TPS1, TPS2, TPSAB1, TPSB1, Tryptase I, Tryptase alpha-1, Tryptase alpha/beta-1, Tryptase-1
Endotoxin level < 10 EU/mg as determined by LAL method.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession Q15661
Form Liquid
Storage buffer 0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Tryptase alpha/beta-1 (β-tryptase/TPSAB1) is a ~30 kDa protein. Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity. Isoform 2 cleaves large substrates, such as fibronectin, more efficiently than isoform 1, but seems less efficient toward small substrates.

1. Jackson, NE. et al. (2008) The Journal of biological chemistry 283, 34178-87. PMID: 18854315
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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