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Anti-Human CD100/SEMA4D Recombinant Antibody (SAA0331), PE (HV563227)

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Anti-Human CD100/SEMA4D Recombinant Antibody (SAA0331), PE

Anti-Human CD100/SEMA4D Recombinant Antibody (SAA0331), PE

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Overview

Catalog No.	HV563227
Description	Anti-Human CD100/SEMA4D Antibody (SAA0331), PE [SAA0331] (HV563227) is a mouse monoclonal antibody detecting SEMA4D in FCM. Suitable for Human. Highlights ●Flow Cytometry — Validated for flow cytometry applications.
Species reactivity	Human
Applications	FCM
Host species	Mouse
Isotype	IgG1, kappa
Clone ID	SAA0331
Conjugation	PE
Clonality	Monoclonal
Target	A8, Semaphorin-4D, GR3, BB18, SEMAJ, C9orf164, CD100, SEMA4D
Endotoxin level	Please contact with the lab for this information.
Accession	Q92854
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Store at 4°C for 12 months. Protect from light. Do not freeze.
Background	Semaphorin-4D (CD100/SEMA4D) is a ~96 kDa protein. Cell surface receptor for PLXNB1 and PLXNB2 that plays an important role in cell-cell signaling. Regulates GABAergic synapse development. Promotes the development of inhibitory synapses in a PLXNB1-dependent manner. Modulates the complexity and arborization of developing neurites in hippocampal neurons by activating PLXNB1 and interaction with PLXNB1 mediates activation of RHOA. Promotes the migration of cerebellar granule cells. 1. Janssen, BJ. et al. (2010) Nature 467, 1118-22. PMID: 20877282 2. Vodrazka, P. et al. (2009) The European journal of neuroscience 30, 1193-208. PMID: 19788569 3. Basile, JR. et al. (2005) Molecular and cellular biology 25, 6889-98. PMID: 16055703 4. Hall, KT. et al. (1996) Proceedings of the National Academy of Sciences of the United States of America 93, 11780-5. PMID: 8876214
Note	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

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Flow CytoMetry

Flow-cytometry using PE anti-human CD100 antibody. Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD100 monoclonal antibody (Catalog: HV563227, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet

Anti-Human CD100/SEMA4D Recombinant Antibody (SAA0331), PE.pdf

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Anti-Human CD100/SEMA4D Recombinant Antibody (SAA0331), PE