Anti-Human CD125/IL5RA Polyclonal Antibody (HX243014)

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50ug
100ug

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HX243014

Description

Anti-Human CD125/IL5RA Polyclonal Antibody (HX243014) is a rabbit polyclonal antibody detecting IL5RA in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CD125/IL5RA (Gly42-Cys182).

Target

IL-5R subunit alpha, IL-5 receptor subunit alpha, IL5RA, IL-5RA, IL-5R-alpha, Interleukin-5 receptor subunit alpha, CDw125, CD125, IL5R

Purification

Purified by antigen affinity column.

Accession

Q01344

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Interleukin-5 receptor subunit alpha (CD125/IL5RA) is a ~47 kDa protein. Cell surface receptor that plays an important role in the survival, differentiation, and chemotaxis of eosinophils. Acts by forming a heterodimeric receptor with CSF2RB subunit and subsequently binding to interleukin-5. In unstimulated conditions, interacts constitutively with JAK2. Heterodimeric receptor activation leads to JAK2 stimulation and subsequent activation of the JAK-STAT pathway.

1. Monahan, J. et al. (1997) Journal of immunology (Baltimore, Md. : 1950) 159, 4024-34. PMID: 9378992

2. Tavernier, J. et al. (1992) Proceedings of the National Academy of Sciences of the United States of America 89, 7041-5. PMID: 1495999

3. Kusano, S. et al. (2012) Protein science : a publication of the Protein Society 21, 850-64. PMID: 22528658

4. Ogata, N. et al. (1998) Blood 91, 2264-71. PMID: 9516124

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Human CD125/IL5RA polyclonal antibody at 1ug/mL on recombinant Human CD125/IL5RA (Catalog No. HX243012).

References

Datasheet