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Anti-Human Coagulation factor III/Tissue factor Recombinant Antibody (SAA0059) (HB058107)

Anti-Human Coagulation factor III/Tissue factor Recombinant Antibody (SAA0059)
Anti-Human Coagulation factor III/Tissue factor Recombinant Antibody (SAA0059)
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Overview
Catalog No.HB058107
Species reactivityHuman
ApplicationsFCM
Host speciesHuman
IsotypeIgG1, kappa
Clone IDSAA0059
Clonality Monoclonal
Target Tissue factor, CD142, F3, Thromboplastin, Coagulation factor III, TF
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession P13726
Form Liquid
Storage buffer 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background

Tissue factor (CD142/F3) is a ~33 kDa protein. Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited proteolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade. CD142 is the therapeutic target of tisotumab vedotin (Tivdak).

Note For research use only.
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  • Anti-Human Coagulation factor III/Tissue factor Recombinant Antibody (SAA0059)

    Flow Cytometry

    Flow-cytometry using anti-human CD142 antibody.CD142 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD142 antibody monoclonal antibody (Catalog # HB058107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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