Anti-Human EMMPRIN/CD147 Recombinant Antibody (4A5) (HW664023)

Name: Anti-Human EMMPRIN/CD147 Recombinant Antibody (4A5)
Brand: abinScience
SKU: HW664023
Price: 110 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD):

Spec:

20ug
50ug
100ug
1mg

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HW664023

Species reactivity

Human

Applications

Host species

Mouse

Isotype

IgG2a, kappa

Clone ID

4A5

Clonality

Monoclonal

Tested applications

WB, FCM

Target

Hepatoma-associated antigen, BSG, HAb18G, 5F7, TCSF, OK blood group antigen, Collagenase stimulatory factor, CD147, Leukocyte activation antigen M6, Basigin, Extracellular matrix metalloproteinase inducer, EMMPRIN, Tumor cell-derived collagenase stimulatory factor

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P35613

RRID

Anti-Human CD147/BSG Antibody (4A5) (abinScience Cat# HW664023, RRID:AB_3728962)
_x005F_x000D_

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Basigin (CD147/BSG) is a ~42 kDa protein. Essential for normal retinal maturation and development. Acts as a retinal cell surface receptor for NXNL1 and plays an important role in NXNL1-mediated survival of retinal cone photoreceptors. In association with glucose transporter SLC16A1/GLUT1 and NXNL1, promotes retinal cone survival by enhancing aerobic glycolysis and accelerating the entry of glucose into photoreceptors. May act as a potent stimulator of IL6 secretion in multiple cell lines that include monocytes.

1. Aït-Ali, N. et al. (2015) Cell 161, 817-32. PMID: 25957687

2. Redzic, JS. et al. (2011) Journal of molecular biology 411, 68-82. PMID: 21620857

Note

For research use only.

Images

Bioactivity

Detects Human CD147/BSG in indirect ELISAs.
SDS-PAGE

SDS-PAGE for Anti-Human CD147/BSG Antibody (4A5).
Flow CytoMetry

Flow-cytometry using FITC anti-human CD147 antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD147 monoclonal antibody (Catalog: HW664023, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-CD147/BSG monoclonal antibody at 1μg/ml on various samples.
Lane 1: recombinant human CD147/BSG (Catalog No: HW664011)