Anti-Human CD300A Polyclonal Antibody (HV400014)

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Overview

Catalog No.

HV400014

Description

Anti-Human CD300A Polyclonal Antibody (HV400014) is a rabbit polyclonal antibody detecting CMRF35-like molecule 8; CLM-8; CD300 antigen-like family member A; CMRF-35-H9; CMRF35-H9; CMRF35-H; IRC1/IRC2; Immunoglobulin superfamily member 12; IgSF12; Inhibitory receptor protein 60; IRp60; NK inhibitory receptor; CD300a; CD300A; CMRF35H; IGSF12; HSPC083 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant human CD300A (Leu18-Pro180).

Target

CMRF35-like molecule 8; CLM-8; CD300 antigen-like family member A; CMRF-35-H9; CMRF35-H9; CMRF35-H; IRC1/IRC2; Immunoglobulin superfamily member 12; IgSF12; Inhibitory receptor protein 60; IRp60; NK inhibitory receptor; CD300a; CD300A; CMRF35H; IGSF12; HSPC083

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q9UGN4

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

CMRF35-like molecule 8 (CD300A) is a ~33 kDa protein. Inhibitory receptor which may contribute to the down-regulation of cytolytic activity in natural killer (NK) cells, and to the down-regulation of mast cell degranulation. Negatively regulates the Toll-like receptor (TLR) signaling mediated by MYD88 but not TRIF through activation of PTPN6.

1. Clark, GJ. et al. (2000) Tissue antigens 55, 101-9. PMID: 10746781

2. Bachelet, I. et al. (2005) Journal of immunology (Baltimore, Md. : 1950) 175, 7989-95. PMID: 16339535

3. Green, BJ. et al. (1998) International immunology 10, 891-9. PMID: 9701027

4. Kim, EJ. et al. (2012) Immunology 135, 226-35. PMID: 22043923

Note

For research use only

Images

References

Datasheet

Anti-Human CD300A Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human CD300A Polyclonal Antibody (HV400014)

Overview

Images

References

Datasheet

SDS

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