Human CD300a Recombinant Protein (C-Fc) (HV400011)

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Catalog No.	HV400011
Description	Recombinant Human CD300a Protein, C-Fc (HV400011) expressed in Mammalian Cells, spanning Met1-Gln178. Purity: >90% by SDS-PAGE. Highlights ●Fc Fusion — Enhanced stability with Protein A/G purification compatibility. ●Mammalian Expression — Proper folding and native glycosylation. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	Mammalian Cells
Accession	Q9UGN4
Protein length	Met1-Gln178
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	45.84 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Inhibitory receptor protein 60, IRp60, CMRF35-H, CLM-8, CMRF35-H9, CMRF35-like molecule 8, CD300A, NK inhibitory receptor, IgSF12, CD300a, CMRF35H, IRC1/IRC2, IGSF12, CD300 antigen-like family member A, CMRF-35-H9, Immunoglobulin superfamily member 12
Background	CMRF35-like molecule 8 (CD300a) is a ~33 kDa protein. Inhibitory receptor which may contribute to the down-regulation of cytolytic activity in natural killer (NK) cells, and to the down-regulation of mast cell degranulation. Negatively regulates the Toll-like receptor (TLR) signaling mediated by MYD88 but not TRIF through activation of PTPN6. 1. Clark, GJ. et al. (2000) Tissue antigens 55, 101-9. PMID: 10746781 2. Bachelet, I. et al. (2005) Journal of immunology (Baltimore, Md. : 1950) 175, 7989-95. PMID: 16339535 3. Green, BJ. et al. (1998) International immunology 10, 891-9. PMID: 9701027 4. Kim, EJ. et al. (2012) Immunology 135, 226-35. PMID: 22043923
Note	For research use only.

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SDS-PAGE for Recombinant Human CD300a Protein, C-Fc

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Datasheet

Human CD300a Recombinant Protein (C-Fc).pdf

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Formula

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Human CD300a Recombinant Protein (C-Fc) (HV400011)

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