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Anti-Human ENO1/Alpha-enolase Monoclonal Antibody (1A067) (HY081025)

Anti-Human ENO1/Alpha-enolase Monoclonal Antibody (1A067)
Anti-Human ENO1/Alpha-enolase Monoclonal Antibody (1A067)
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Overview
Catalog No.HY081025
Description
Anti-Human ENO1/Alpha-enolase Monoclonal Antibody (1A067) [1A067] (HY081025) is a mouse monoclonal antibody detecting Alpha-enolase in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Recombinant — Superior batch-to-batch reproducibility with defined sequences.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesMouse
ClonalityMonoclonal
IsotypeIgG1
Clone ID1A067
Immunogen Mammalian cells - derived recombinant Human ENO1 / Alpha-enolase (Met1-Lys434).
Target Non-neural enolase, 2-phospho-D-glycerate hydro-lyase, MPB-1, ENO1, Plasminogen-binding protein, Enolase 1, C-myc promoter-binding protein, Alpha-enolase, MBP-1, MBPB1, MPB1, ENO1L1, NNE, Phosphopyruvate hydratase,
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession P06733
Form Liquid
Storage buffer 0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Alpha-enolase (ENO1) is a ~47 kDa protein. Enolase that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in glycolysis and the reverse reaction in gluconeogenesis. Also involved in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.

1. Sugahara, T. et al. (1992) Cytotechnology 10, 137-46. PMID: 1369209
2. Huang, H. et al. (2018) Molecular cell 70, 663-678.e6. PMID: 29775581
3. Feo, S. et al. (2000) FEBS letters 473, 47-52. PMID: 10802057
4. López-Alemany, R. et al. (2003) American journal of hematology 72, 234-42. PMID: 12666133
5. Ray, R. et al. (1991) Molecular and cellular biology 11, 2154-61. PMID: 2005901
Note For research use only.
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  • Anti-Human ENO1/Alpha-enolase Monoclonal Antibody (1A067)

    SDS-PAGE

    SDS-PAGE for Anti-Human ENO1/Alpha-enolase Monoclonal Antibody (1A067)

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
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