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Anti-Human F7/Coagulation factor VII Recombinant Antibody (3A4) (HY060023)

Anti-Human F7/Coagulation factor VII Recombinant Antibody (3A4)
Anti-Human F7/Coagulation factor VII Recombinant Antibody (3A4)
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Overview
Catalog No.HY060023
Species reactivityHuman
ApplicationsELISA, WB
Host speciesMouse
IsotypeIgG2a, kappa
Clone ID3A4
Clonality Monoclonal
Target F7, Coagulation factor VII, FVII, Proconvertin, Serum prothrombin conversion accelerator, SPCA, Eptacog alfa
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession P08709
RRID Anti-Human F7/Coagulation factor VII Antibody (3A4) (abinScience Cat# HY060023, RRID:AB_3727167)
Form Liquid
Storage buffer 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:2000-1:20000
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background

Coagulation factor VII (F7) is a ~51 kDa protein. Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa also converts factor IX to factor IXa in the presence of tissue factor and calcium.

1. Osterud, B. et al. (1977) Proceedings of the National Academy of Sciences of the United States of America 74, 5260-4. PMID: 271951
Note For research use only.
Images
  • Anti-Human F7/Coagulation factor VII Recombinant Antibody (3A4)

    SDS-PAGE

    SDS-PAGE for Anti-Human F7/Coagulation factor VII Antibody (3A4).

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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