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Anti-Human Fibronectin Recombinant Antibody (L19) (HY259043)

Anti-Human Fibronectin Recombinant Antibody (L19)
Anti-Human Fibronectin Recombinant Antibody (L19)
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Overview
Catalog No.HY259043
Species reactivityHuman
ApplicationsELISA
Host speciesHuman
IsotypescFv
Clone IDL19
Clonality Monoclonal
Target Fibronectin, FN, Cold-insoluble globulin, CIG, Anastellin, Ugl-Y1, Ugl-Y2, Ugl-Y3, FN1, FN
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession P02751
Form Liquid
Storage buffer 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:2000-1:20000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Fibronectin is a ~272 kDa protein. Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts. Acts as a ligand for the LILRB4 receptor, inhibiting FCGR1A/CD64-mediated monocyte activation.

1. Owens, RJ. et al. (1986) The EMBO journal 5, 2825-30. PMID: 3024962
2. Garcia-Pardo, A. et al. (1987) The Biochemical journal 241, 923-8. PMID: 3593230
3. Calaycay, J. et al. (1985) The Journal of biological chemistry 260, 12136-41. PMID: 3900070
4. Rostagno, A. et al. (1994) The Journal of biological chemistry 269, 31938-45. PMID: 7989369
5. Su, MT. et al. (2021) International immunology 33, 447-458. PMID: 34089617
Note For research use only
Images
  • Anti-Human Fibronectin Recombinant Antibody (L19)

    SDS-PAGE

    SDS-PAGE for Anti-Human Fibronectin Antibody (L19)

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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