Anti-Human Fibronectin Recombinant Antibody (SAA0522) (HY259013)

Name: Anti-Human Fibronectin Recombinant Antibody (SAA0522)
Brand: abinScience
SKU: HY259013
Price: 126 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HY259013

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IF, IHC, SPR, WB, FCM

Host species

Mouse

Isotype

IgG2a, kappa

Clone ID

SAA0522

Clonality

Monoclonal

Tested applications

FCM

Target

Cold-insoluble globulin, CIG, FN, Fibronectin, FN1

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P02751

RRID

Anti-Human FN1/Fibronectin Antibody (SAA0522) (abinScience Cat# HY259013, RRID:AB_3726039)

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution

Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Note

For research use only.

Isotype Control

Mouse IgG2a, kappa Recombinant Isotype Control Antibody (MOPC-173) (MF533507)

Images

SDS-PAGE

SDS-PAGE for Anti-Human FN1/Fibronectin Antibody (SAA0522)
Flow cytometry

Flow-cytometry using anti-human FN1 antibody. HepG2 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human FN1 monoclonal antibody (Catalog HY259013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet

Anti-Human Fibronectin Recombinant Antibody (SAA0522).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human Fibronectin Recombinant Antibody (SAA0522) (HY259013)

Overview

Images

References

Datasheet

SDS

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