









| Catalog No. | HT173013 | ||||
|---|---|---|---|---|---|
| Species reactivity | Human | ||||
| Applications | Blocking, ELISA, FCM | ||||
| Host species | Mouse | ||||
| Isotype | IgG2a, kappa | ||||
| Clone ID | G9.2-17 | ||||
| Clonality | Monoclonal | ||||
| Target | Ecalectin, LGALS9, Galectin-9, Gal-9, Tumor antigen HOM-HD-21 | ||||
| Endotoxin level | Please contact with the lab for this information. | ||||
| Purity | >95% as determined by SDS-PAGE. | ||||
| Purification | Protein A/G purified from cell culture supernatant. | ||||
| Accession | O00182 | ||||
| RRID | Anti-Human Gal-9/LGALS9 Antibody (G9.2-17) (abinScience Cat# HT173013, RRID:AB_3725834) |
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| Form | Liquid | ||||
| Storage buffer | 0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
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| Product Usage Information |
| ||||
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. | ||||
| Note | For research use only. |

Flow-cytometry using anti-human Galectin-9 antibody.Human Jurkat cell line were stained with an irrelevant antibody (Blue Histogram) or an anti-human Galectin-9 antibody monoclonal antibody (Catalog # HT173013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using anti-human Galectin-9 antibody.MOLT-4 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human Galectin-9 antibody monoclonal antibody (Catalog # HT173013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using anti-human Galectin-9 antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human Galectin-9 antibody monoclonal antibody (Catalog # HT173013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

SDS-PAGE for Anti-Human Gal-9/LGALS9 Antibody (G9.2-17).

Flow-cytometry using anti-human Galectin-9 antibody. THP-1 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an anti-human Galectin-9 monoclonal antibody (Catalog HT173013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.




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