Anti-Human HCRTR1 Polyclonal Antibody (HT363014)

Name: Anti-Human HCRTR1 Polyclonal Antibody
Brand: abinScience
SKU: HT363014
Price: 168 USD
Availability: InStock

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SDS

Overview

Catalog No.

HT363014

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human HCRTR1 (Gln179-Lys213).

Target

Hypocretin receptor type 1, Ox1-R, Ox1R, Ox-1-R, Orexin receptor type 1, HCRTR1

Purification

Purified by antigen affinity column.

Accession

O43613

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Orexin/Hypocretin receptor type 1 (HCRTR1/OX1R) is a ~47 kDa protein. G-protein coupled receptor that binds the neuropeptide orexin-A with high affinity, and orexin-B with lower affinity, two peptides derived from a common precursor, prepro-orexin. Its activity is mediated via a G(q)-protein-coupled pathway, which activates the phosphatidylinositol-calcium second messenger system in response to orexin-A binding. In addition to G(q)-mediated signaling, orexin-A stimulation also promotes beta-arrestin recruitment, leading to receptor internalization. Plays a significant role in the regulation of food intake.

1. Hellmann, J. et al. (2020) Proceedings of the National Academy of Sciences of the United States of America 117, 18059-18067. PMID: 32669442

2. Sakurai, T. et al. (1998) Cell 92, 573-85. PMID: 9491897

3. Milasta, S. et al. (2005) The Biochemical journal 387, 573-84. PMID: 15683363

Note

For research use only.

Images

References

Datasheet

Anti-Human HCRTR1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human HCRTR1 Polyclonal Antibody (HT363014)

Overview

Images

References

Datasheet

SDS

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