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Anti-Human HGF Recombinant Antibody (SFN68) (HB191013)

Anti-Human HGF Recombinant Antibody (SFN68)
Anti-Human HGF Recombinant Antibody (SFN68)
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Overview
Catalog No.HB191013
Description
Anti-Human HGF Recombinant Antibody (SFN68) (HB191013) is a mouse monoclonal antibody detecting HGF in Blocking, ELISA. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsBlocking, ELISA
Host speciesMouse
ClonalityMonoclonal
IsotypeIgG2a, kappa
Clone IDSFN68
Target Hepatocyte growth factor, Hepatopoietin-A, Scatter factor, SF, Hepatocyte growth factor alpha chain, Hepatocyte growth factor beta chain, HGF, HPTA
Endotoxin level < 10 EU/mg as determined by LAL method.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession P14210
Form Liquid
Storage buffer 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:2000-1:20000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background

Hepatocyte growth factor (SF,HPTA,Hepatopoietin-A,Hepatocyte growth factor,Scatter factor,HGF) is a ~83 kDa protein. Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization. Activates MAPK signaling following TMPRSS13 cleavage and activation.

1. Tolbert, WD. et al. (2010) Proceedings of the National Academy of Sciences of the United States of America 107, 13264-9. PMID: 20624990
2. Stamos, J. et al. (2004) The EMBO journal 23, 2325-35. PMID: 15167892
3. Hashimoto, T. et al. (2010) The FEBS journal 277, 4888-900. PMID: 20977675
Note For research use only.
Isotype Control Mouse IgG2a (Fc Silence) Recombinant Isotype Control (MOPC 173) (MF533023)
Images
  • Anti-Human HGF Recombinant Antibody (SFN68)

    SDS-PAGE

    SDS-PAGE for Anti-Human HGF Antibody (SFN68).

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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