Anti-Mouse CD274/PD-L1/B7-H1 Recombinant Nanobody (C3) (MV974033)

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50ug
100ug
1mg

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

MV974033

Description

Anti-Mouse CD274/PD-L1/B7-H1 Nanobody (C3) [C3] (MV974033) is a alpaca monoclonal antibody detecting B7-H1 in ELISA, FuncS. Suitable for Mouse.

Species reactivity

Mouse

Applications

ELISA, FuncS, WB

Host species

Alpaca

Isotype

VHH-8His-Cys-tag

Clone ID

Clonality

Monoclonal

Tested applications

Target

B7 homolog 1, CD274, Pdcd1lg1, PDCD1 ligand 1, B7h1, Programmed death ligand 1, B7-H1, PD-L1, Cd274, Pdl1, Programmed cell death 1 ligand 1, Pdcd1l1

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Purified by Nickel column.

Accession

Q9EP73

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Programmed cell death 1 ligand 1 (CD274/PD-L1) is a ~32 kDa protein. Plays a critical role in induction and maintenance of immune tolerance to self. As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response. Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10). CD274 is the therapeutic target of atezolizumab (Tecentriq) and durvalumab (Imfinzi).

1. Tamura, H. et al. (2001) Blood 97, 1809-16. PMID: 11238124

2. Freeman, GJ. et al. (2000) The Journal of experimental medicine 192, 1027-34. PMID: 11015443

3. Wang, S. et al. (2003) The Journal of experimental medicine 197, 1083-91. PMID: 12719480

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Mouse CD274/PD-L1/B7-H1 Nanobody (C3)
Bioactivity

Detects recombinant mouse PDL1 (Catalog No: MV974011) in indirect ELISA.
Western blot

Western blot analysis was performed using anti-PDL1 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant mouse PDL1 (Catalog No: MV974011)