Anti-Mouse IFNG/IFN-gamma Polyclonal Antibody (MF813014)

Name: Anti-Mouse IFNG/IFN-gamma Polyclonal Antibody
Brand: abinScience
SKU: MF813014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

MF813014

Description

Anti-Mouse IFNG/IFN-gamma Polyclonal Antibody (MF813014) is a rabbit polyclonal antibody detecting IFN-gamma in ELISA, IHC, WB. Suitable for Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant mouse IFNG/IFN-gamma (His23-Cys155).

Target

IFN-gamma, Ifng, Interferon gamma

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P01580

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Interferon gamma is a ~17 kDa protein. Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation. Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription. Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits.

1. Pearl, JE. et al. (2001) Cellular immunology 211, 43-50. PMID: 11585387

2. Huang, S. et al. (1993) Science (New York, N.Y.) 259, 1742-5. PMID: 8456301

3. Baldridge, MT. et al. (2010) Nature 465, 793-7. PMID: 20535209

4. Matatall, KA. et al. (2014) Stem cells (Dayton, Ohio) 32, 3023-30. PMID: 25078851

Note

For research use only

Images

References

Datasheet