Anti-TDP43 Polyclonal Antibody (HC445014)

Name: Anti-TDP43 Polyclonal Antibody
Brand: abinScience
SKU: HC445014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HC445014

Description

Anti-TDP43 Polyclonal Antibody (HC445014) is a rabbit polyclonal antibody detecting TDP43 in ELISA, IHC, WB. Suitable for Human, Mouse, Chicken, Pongo abelii, and Xenopus tropicalis.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human TARDBP (Ser104-Pro262).

Target

TAR DNA-binding protein 43, TARDBP, TDP-43, TDP43

Purification

Purified by antigen affinity column.

Accession

Q13148

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

TAR DNA-binding protein 43 (TARDBP/TDP43) is a ~44 kDa protein. RNA-binding protein that is involved in various steps of RNA biogenesis and processing. Preferentially binds, via its two RNA recognition motifs RRM1 and RRM2, to GU-repeats on RNA molecules predominantly localized within long introns and in the 3'UTR of mRNAs. In turn, regulates the splicing of many non-coding and protein-coding RNAs including proteins involved in neuronal survival, as well as mRNAs that encode proteins relevant for neurodegenerative diseases. Plays a role in maintaining mitochondrial homeostasis by regulating the processing of mitochondrial transcripts. Also regulates mRNA stability by recruiting CNOT7/CAF1 deadenylase on mRNA 3'UTR leading to poly(A) tail deadenylation and thus shortening.

1. Bhardwaj, A. et al. (2013) Nucleic acids research 41, 5062-74. PMID: 23519609

2. Lukavsky, PJ. et al. (2013) Nature structural & molecular biology 20, 1443-9. PMID: 24240615

3. Kuo, PH. et al. (2014) Nucleic acids research 42, 4712-22. PMID: 24464995

4. Tollervey, JR. et al. (2011) Nature neuroscience 14, 452-8. PMID: 21358640

5. Wang, A. et al. (2018) The EMBO journal 37. PMID: 29438978

6. Izumikawa, K. et al. (2017) Scientific reports 7, 7709. PMID: 28794432

7. Fukushima, M. et al. (2019) FEBS letters 593, 277-287. PMID: 30520513

8. Colombrita, C. et al. (2009) Journal of neurochemistry 111, 1051-61. PMID: 19765185

9. Higashi, S. et al. (2013) Journal of neurochemistry 126, 288-300. PMID: 23398327

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-TDP43 polyclonal antibody at 1ug/mL on recombinant TDP43 (Catalog No. HC445012).

References

Datasheet

Anti-TDP43 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-TDP43 Polyclonal Antibody (HC445014)

Overview

Images

References

Datasheet

SDS

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