Human TDP43 Recombinant Protein (N-His) (HC445022)

Name: Human TDP43 Recombinant Protein (N-His)
Brand: abinScience
SKU: HC445022
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HC445022
Description	Recombinant Human TDP43 Protein, N-His (HC445022) expressed in E. coli, spanning Ser2-Met414. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q13148
Protein length	Ser2-Met414
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	45.85 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	TAR DNA-binding protein 43, TARDBP, TDP-43, TDP43
Background	TAR DNA-binding protein 43 (TDP43) is a ~44 kDa protein. RNA-binding protein that is involved in various steps of RNA biogenesis and processing. Preferentially binds, via its two RNA recognition motifs RRM1 and RRM2, to GU-repeats on RNA molecules predominantly localized within long introns and in the 3'UTR of mRNAs. In turn, regulates the splicing of many non-coding and protein-coding RNAs including proteins involved in neuronal survival, as well as mRNAs that encode proteins relevant for neurodegenerative diseases. Plays a role in maintaining mitochondrial homeostasis by regulating the processing of mitochondrial transcripts. Also regulates mRNA stability by recruiting CNOT7/CAF1 deadenylase on mRNA 3'UTR leading to poly(A) tail deadenylation and thus shortening. 1. Bhardwaj, A. et al. (2013) Nucleic acids research 41, 5062-74. PMID: 23519609 2. Lukavsky, PJ. et al. (2013) Nature structural & molecular biology 20, 1443-9. PMID: 24240615 3. Kuo, PH. et al. (2014) Nucleic acids research 42, 4712-22. PMID: 24464995 4. Tollervey, JR. et al. (2011) Nature neuroscience 14, 452-8. PMID: 21358640 5. Wang, A. et al. (2018) The EMBO journal 37. PMID: 29438978 6. Izumikawa, K. et al. (2017) Scientific reports 7, 7709. PMID: 28794432 7. Fukushima, M. et al. (2019) FEBS letters 593, 277-287. PMID: 30520513 8. Colombrita, C. et al. (2009) Journal of neurochemistry 111, 1051-61. PMID: 19765185 9. Higashi, S. et al. (2013) Journal of neurochemistry 126, 288-300. PMID: 23398327
Note	For research use only.